Universal Pathogen
DNA/RNA Extraction Kit
Quick Start Guide
magenti Universal Pathogen DNA/RNA Extraction Kit
Lysis / Binding Buffer (600µl)
Columns 1 & 7 Binding magnetic nanoparticles (200µl)
Columns 2 & 8
Wash #1 Buffer (600µl)
Columns 3 & 9
Wash #2 Buffer (600µl)
Columns 4 & 10
Elution Buffer (90µl)
Columns 6 & 12
| 200μl sample added to Lysis/ Binding Buffer | 90μl extracted and purified RNA elution | 200μl sample added to Lysis/ Binding Buffer | 90μl extracted and purified RNA elution |
UPKit miQron protocol parameters
| Step Name | Column | Volume (μ1) | Time (sec) | Mixing Speed (1-10) | Dry Time (sec) | Magnet Capture Time (sec) |
| Lysis | 1 & 7 | 800 | 300 | 7 | 0 | – |
| Bead Transfer | 2 & 8 | 200 | 30 | 5 | 0 | 30 |
| Binding | 1 & 7 | 800 | 300 | 5 | 0 | 30 |
| Wash #1 | 3 & 9 | 600 | 60 | 6 | 0 | 60 |
| Wash #2 | 4 & 10 | 600 | 60 | 6 | 60 | 60 |
| Elution | 6 & 12 | 90 | 120 | 7 | 0 | 60 |
| Discard Comb | 2 & 8 | 600 | – | 5 | – | – |
| 1. Remove the protective foil. | 2. Add up to 16 samples to Lysis/Binding Buffer (columns 1 and 7). | 3. Place plate into the miQron, taking care that the label is facing outward. |
 |  |  |
| 4. Insert two combs. | 5. Select the VRKit protocol and press | Columns 5 and 11 contain the purified RNA elution. |
 |  When program is complete, remove plate from miQron and discard combs. |  |
UP Universal Pathogen DNA/RNA
Extraction Kit
VR0016-12
1.0
