Plant RNA Extraction Kit
Quick Start Guide
magnetiQ Plant RNA Extraction Kit
Binding Buffer (650μl)
Columns 1 & 7
Wash #1 Buffer (600μl)
Columns 2 & 8
Wash #2 Buffer (600μl)
Columns 3 & 9, 4 & 10
Elution Buffer (100μl)
Columns 5 & 11
| 300μl sample added to Binding Buffer | 100μl extracted and purified RNA elution | 300μl sample added to Binding Buffer | 100μl extracted and purified RNA elution |
PRKit–A miQron protocol parameters
| Step Name | Column | Volume (pi) | Time (sec) | Mixing Speed (1-10) | Dry Time (sec) | Magnet Capture Time (sec) |
| Binding | 1 & 7 | 650 | 300 | 7 | 0 | 150 |
| Wash #1 | 2 & 8 | 600 | 60 | 7 | 0 | 90 |
| Wash #2 | 3 & 9 | 600 | 60 | 7 | 0 | 90 |
| Wash #2 | 4 & 10 | 600 | 60 | 7 | 300 | 90 |
| Elution | 5 & 11 | 50 | 60 | 10 | 0 | 150 |
| Discard Comb | 2& 8 | 600 | 0 | 5 | 0 | 0 |
| 1. Add up to 50mg of fresh plant leaves sample to the lysis bead tube provided. | 1. Add 600μl Lysis Buffer, and 60μl PPB. Mix for 10 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins. |
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| 3. To the RNA Extraction Kit Plate A transfer up to 300μl of supernatant to Binding Buffer #1 (columns 1 & 7). You can add up to 16 samples. | 4. Place plate into the miQron, taking care that the label is facing outward. |
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| 5. Insert two combs. | 6. Select PRKit – Part A and press |
 |  When program is complete, remove plate from miQron and discard combs. |
Proceed to Part B DNase Treatment
| 7. Add 100μl of DNase Reaction BuFFer to DNase pellet and then add 100μl of glycerol. Mix by gently inverting the tube. Reconstituted pellet must be stored at -20 °C. | 8. For each RNA sample, prepare DNase BuFFer by adding 10μl of DNase prepared in the previous step to 40μl of DNase Reaction BuFFer in a microfuge tube. Mix DNase with buFFer by gently inverting the tube a few times. |
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| 9. To the RNA Extraction Kit Plate add 50μl of DNase BuFFer to Elution BuFFer (columns 5 & 11). Dispense directly into the elution buFFer not on the walls. If there are any droplets of elution buFFer left on the walls, slide them to the well bottom by gently shaking or tapping the plate on a solid surface. | 10. Gently shake Plate A for 10 seconds by hand and incubate at room temp for 20 mins. From the RNA Extraction Kit Plate A transfer 100uL of the Elution BuFFer (columns 5 & 11) to each well (columns 1 & 7) of the RNA Extraction Kit Plate B |
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| 11. Place Plate B into the miQron, taking care that the label is facing outward. | 12. Insert two combs. |
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| 13. Select PRKit – Part B and press | Columns 5 and 11 contain the purified RNA elution. |
| When program is complete, remove plate from miQron and discard combs. |  |
PR Plant RNA Extraction Kit
PR 0016-12
1.0
