WW0016-12 Wastewater
DNA and RNA Extraction Kit
User Guide
WW0016-12 Wastewater DNA and RNA Extraction Kit
WWKit miQron protocol parameters
| Step Name | Column | Volume (μ l) | Time (sec) | Mixing Speed (1–10) | Dry Time (sec) | Magnet Capture Time (sec) | Temp |
| Load | |||||||
| Bead Pickup | 2 & 8 | 30 | 60 | Medium | 0 | 1 x 30 sec | OFF |
| Binding | 1 & 7 | 1000 | 300 | Medium | 0 | 5 x 30 sec | OFF |
| Wash #1 | 3 & 9 | 600 | 60 | Medium | 0 | 2 x 30 sec | OFF |
| Wash #2 | 4 & 10 | 600 | 60 | Medium | 120 | 2 x 30 sec | OFF |
| Elution | 6 & 12 | 100 | 300 | Low | 0 | 2 x 30 sec | 65ºC |
| -Unload- | 2 & 8 | ||||||
- Transfer 10ml of wastewater sample to 15ml conical tube. Add 100μl of Concentration Buffer to sample. Invert 5 times.
Add 75μl of Concentration Beads. Invert 5 times and incubate for 10 mins. Invert 3 more times at the 5 min mark.
- Place sample on 15ml magnetic rack to capture beads, then discard supernatant.
Remove tube from magnetic rack.
- Resuspend beads in 1ml RNase-free water, then transfer the mixture to a clean 2ml centrifuge tube.
Place tube on 2ml magnetic rack to capture beads, then discard supernatant.
- Remove tube from magnetic rack and resuspend beads in 400μl of Lysis Buffer.
Vortex at max speed for 5 mins, then separate beads using a 2ml magnetic rack.
- Avoiding pellet, transfer up to 400μl of supernatant to the Binding Buffer wells (Columns 1 & 7).
You can add up to 16 samples.
- Place plate into the miQron, taking care that the label is facing outward.
- Insert two combs.
- Select the WWKit protocol and press
When program is complete, remove plate from miQron and discard combs.
Columns 6 & 12 contains the purified DNA/RNA elution.
When program is complete, remove plate from miQron and discard combs.
WW Wastewater DNA/RNA Extraction Kit|
WW0016-12
