Galenvs 20220407 Viral Rna Extraction Kit User Guide

Viral RNA
Extraction Kit
Quick Start Guide

GALENVS 20220407 Viral RNA Extraction Kit

Before first use, add ethanol (>95%) to Wash Buffer #2 as per label instructions.
Prior to extraction, mix bottles well by inverting upside down several times.

1. In 1.5ml microfuge tube, add 100μl of sample (swab, saliva, cell-free body fluids, viral transportation media (VTM), inactive transport media (ITM), culture supernatants,or bronchoalveolar lavage fluid).
2. Add 400μl of Lysis/Binding Buffer and mix well by pipetting up-down 10–15x.
   Incubate 5 mins to allow for lysis and RNA binding.
3. Place tube on magnetic rack for 1–2 mins to capture RNA-bead complex, then discard supernatant.
4. Remove tube from magnetic rack and resuspend RNA–bead complex in 600μl of Wash Buffer #1.
   Return to magnetic rack for 1–2 mins, then discard supernatant.
5. Repeat wash with 600μl of Wash Buffer #2, return to magnetic rack for 1–2 mins, then discard supernatant and leave to dry for 1 min.
6. Remove tube from magnetic rack and resuspend RNA–bead complex in 50μl of Elution Buffer.
   Mix well by pipetting up-down 15–20x to elute RNA from beads and let stand for 1–2 mins.
7. Place tube on magnetic rack to separate beads (~1–2 mins).
8. Transfer clean RNA solution (supernatant) to clean tube.

VR Viral RNA Extraction Kit
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