Galenvs Magenti Soil Dna Extraction Kit User Guide

GALENVS magenti Soil DNA Extraction Kit

PARTS

METHOD

1. Add up to 250mg of soil sample to lysis bead tube provided. Add 900µl of Lysis Buffer, mix for 10 mins using TissueLyser at max speed, or vortex for 20 mins, then centrifuge at 20,000g for 1 min.
   For manure sample: in addition to the above, heat mixture at 65ºC for 10 mins before proceeding to step 2.
2. Avoiding pellet, transfer up to 400–500µl of supernatant to clean centrifuge tube.
   Add 200μl of IR buffer, vortex for 10–20s, then centrifuge for 1 min.
3. Remove the protective foil.
4. Avoiding pellets, transfer up to 300μl of supernatant to Binding Buffer (Columns 1 & 7).
   You can add up to 16 samples.
5. Place plate into the microns, taking care that the label is facing outward.
6. Insert two combs.
7. Select the SD Kit protocol and pressColumns 5 and 11 contain the purified DNA elution.