Viral RNA Extraction Kit
Quick Start Guide
Lysis / Binding Buμer (800μl)
Columns 1 & 7
Wash #1 Buμer (600μl)
Columns 2 & 8μ
Wash #2 Buμer (600μl)
Columns 3 & 9
Elution Buμer (100μl)
Columns 5 & 11
| 200μl sample added to Lysis/ Binding Buffer | 90μl extracted and purified RNA elution | 200μl sample added to Lysis/ Binding Buffer | 90μl extracted and purified RNA elution |
VRKit miQron protocol parameters
| Step Name | Column | Volume (p1) | Time (sec) | Mixing Speed (1-10) | Dry Time (sec) | Magnet Capture Time (sec) |
| Lysis / Binding | 1 & 7 | 800 | 300 | 5 | 0 | 180 |
| Wash #1 | 2 & 8 | 600 | 60 | 5 | 0 | 120 |
| Wash #2 | 3 & 9 | 600 | 60 | 5 | 60 | 120 |
| Elution | 5 & 11 | 90 | 60 | 5 | 0 | 90 |
| Discard comb | 2& 8 | 600 | 0 | 5 | 0 | 0 |
| 1. Remove the protective foil. | 2. Add up to 16 samples to Lysis/Binding Buffer (columns 1 and 7). | 3. Place plate into the miQron, taking care that the label is facing outward. |
 |  |  |
| 4. Insert two combs. | 5. Select the VRKit protocol and press | Columns 5 and 11 contain the purified RNA elution. |
 |  When program is complete, remove plate from miQron and discard combs. |  |
VR Viral RNA Extraction Kit
VR0016-12
1.0
