PD0016-12 Plant DNA Extraction Kit
User Guide
PD0016-12 Plant DNA Extraction Kit
PDKit miQron protocol parameters
| Step Name | Column | Volume ( μl) | Time (sec) | Mixing Speed (1–10) | Dry Time (sec) | Magnet Capture Time (sec) |
| Binding | 1 & 7 | 650 | 300 | 7 | 0 | 150 |
| Wash #1 | 2 & 8 | 600 | 60 | 7 | 0 | 90 |
| Wash #2 | 3 & 9 | 600 | 60 | 7 | 0 | 90 |
| Wash #2 | 4 & 10 | 600 | 60 | 7 | 300 | 90 |
| Elution | 5 & 11 | 100 | 60 | 10 | 0 | 150 |
| Discard Comb | 2 & 8 | 600 | 0 | 5 | 0 | 0 |
- Add up to 50mg of plant leaves sample to the lysis bead tube provided.
- Add 600μl Lysis Buffer, 60μl PPB, and 5μl RNase.
Mix for 3 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.
- Remove the protective foil.
- Avoiding pellets, transfer up to 300-400μl of supernatant to Binding Buffer (Columns 1 & 7).
You can add up to 16 samples.
- Place plate into the miQron, taking care that the label is facing outward.
- Insert two combs.
- Select the PDKit protocol and press
When program is complete, remove plate from miQron and discard combs.
Columns 5 and 11 contain the purified DNA elution.
PD Plant DNA Extraction Kit
PD0016-12
When program is complete, remove plate from miQron and discard combs.
PD Plant DNA Extraction Kit
