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2 Mechanical Pipette – HiPette User Manual

HiPette Mechanical Pipette

Mechanical Pipette – HiPette User Manual

Product Information:

The HiPette is a mechanical pipette that comes with adjustable
volume and is fully autoclavable. It is available in different
volume ranges, from 0.1-2.5L to 2000-10000L, and with varying
increments from 0.005L to 10L. The HiPette comes with different tip
sizes, ranging from 10L to 200,300,350L, depending on the volume
range. The product package includes a pipette, calibration/remove
tool, user manual, pipette holder, tips, silicone grease, quality
control certificate, and filter element (for 0.1ul-2.5ul and
0.5ul-10ul volume pipette without filter).

The HiPette is fully autoclavable under 1211atm for 20 mins. It
is recommended to wrap the pipette by gauze before autoclaving and
leave it for 12 hours to dry before use. Greasing both sealing ring
and piston after every 10 times autoclaving is also
recommended.

Product Usage Instructions:

Unpacking: Open the product package and verify
that all the items are present. The package should contain a
pipette, calibration/remove tool, user manual, pipette holder,
tips, silicone grease, quality control certificate, and filter
element (for 0.1ul-2.5ul and 0.5ul-10ul volume pipette without
filter).
Installing the Pipette Holder: Clean the shelf
surface with ethanol before installing the holder. Remove the
protective paper from the adhesive tape and install the holder as
described in Figure 2A. Make sure the holder is pressed against the
edge of the shelf. Place the pipette onto the holder as shown in
Figure 2B.
Pipette Components: Familiarize yourself with
the different components of the pipette, as shown in Figure 4.
Pipette Operation:
1. Volume Setting: The volume of the pipette is
  shown through the handle grip window. To set the delivery volume
  (variable volume pipette only), turn the thumb button clockwise or
  anticlockwise. Make sure that the desired delivery volume clicks
  into place, the digits are completely visible in the display
  window, and the selected volume is within the pipette’s specified
  range. Using excessive force to turn the push button outside the
  range may jam the pipette.
2. Aspiration: Attach a tip to the end of the
  pipette and place it into the liquid to be aspirated. Depress the
  plunger to the first stop and hold it in place while immersing the
  tip into the liquid.
3. Dispensing: Slowly release the plunger to
  dispense the liquid. For variable volume pipettes, make sure to
  release the plunger at a constant speed to ensure accurate
  dispensing.
4. Ejecting the Tip: After dispensing, eject the
  tip by pushing the ejector button located on the side of the
  pipette. Dispose of used tips properly.
Performance Test and Recalibration: Refer to
sections 9.1 and 9.2 of the user manual for instructions on how to
perform a performance test and recalibration.
Maintenance: Regularly clean the pipette with
ethanol and follow the in-house maintenance procedure described in
section 10.2 of the user manual. Grease both sealing ring and
piston after every 10 times autoclaving.
Troubleshooting: Refer to section 11 of the
user manual for troubleshooting tips.
Warranty Information: Refer to section 12 of
the user manual for warranty information.
Specifications: Refer to section 13 of the
user manual for product specifications.

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Mechanical Pipette
HiPette User Manual
12301725 Ver.20211213

CONTENTS
1.YOUR NEW PIPETTE …………………………………………………………………………… 1 1.1 ADJUSTABLE VOLUME PIPETTES ……………………………………………………………… 1 1.2 FULLY AUTOCLAVABLE ………………………………………………………………………….. 2
2.UNPACKING………………………………………………………………………………………… 2 3.INSTALLING THE PIPETTE HOLDER …………………………………………………….. 3 4.PIPETTE COMPONENTS ……………………………………………………………………… 4 5.PIPETTE OPERATION ………………………………………………………………………….. 5
5.1 VOLUME SETTING ……………………………………………………………………………….. 5 5.2 RANGE LOCK …………………………………………………………………………………….. 5 5.3 SEALING AND EJECTING TIPS ………………………………………………………………….. 6 6.PIPETTING TECHNIQUES ……………………………………………………………………. 6 6.1 FORWARD PIPETTING …………………………………………………………………………… 7 6.2 REVERSE PIPETTING ……………………………………………………………………………. 7 7.PIPETTING RECOMMENDATIONS ………………………………………………………… 8 8.STORAGE …………………………………………………………………………………………… 9

9.PERFORMANCE TEST AND RECALIBRATION ……………………………………….. 9 9.1 PERFORMANCE TEST (CHECKING CALIBRATION)…………………………………………. 10 9.2 RECALIBRATION PROCEDURE ……………………………………………………………….. 11
10.MAINTENANCE………………………………………………………………………………… 11 10.1 CLEANING YOUR PIPETTE…………………………………………………………………… 12 10.2 IN-HOUSE MAINTENANCE …………………………………………………………………… 13
11.TROUBLE SHOOTING ………………………………………………………………………. 13 12.WARRANTY INFORMATION ………………………………………………………………. 15 13.SPECIFICATIONS …………………………………………………………………………….. 16

1.YOUR NEW PIPETTE

Your new mechanical pipette is a general purpose pipette for the accurate and precise sampling and dispensing of liquid volumes. The pipettes operate on the air displacement principle and disposable tips. The pipettes cover a volume range from 0.1µL to 10mL. All pipettes have been quality tested according to ISO8655/DIN12650. The quality control according to ISO8655/DIN12650 involves gravimetric testing of each pipette with distilled water (quality 3, DIN ISO 3696) at 22 using the manufacturer’s original tips.
1.1Adjustable volume pipettes

Volume Range 0.1-2.5L 0.5-10L 2-20L 5-50L 10-100L

Increment 0.005L 0.01L 0.02L 0.05L 0.1L

Tips 10L 10L 200,300L 200,300L 200,300,350L

20-200L 30-300L 100-1000L 1000-5000L 2000-10000L

0.2L 0.2L 1L 5L 10L

200,300,350L 200,300,350L 1000L 5mL 10mL

1.2Fully autoclavable
HiPette can be fully autoclavable under 1211atm for 20 mins, Wrap the pipette by gauze before autoclave. It must be left for 12 hours to dry before use. Greasing both sealing ring and piston after every 10 times autoclaving.

2.UNPACKING
The pipette packing contains the following items Pipette Calibration/ remove tool User manual Pipette holder
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Tips Silicone Grease Quality control certificate Filter element0.1ul-2.5ul0.5ul-10ul volume pipette without filter
3.INSTALLING THE PIPETTE HOLDER
For convenience and safety always keep the pipette vertically on its own holder when not in use. When installing the holder, please follow the instruction below: 1. Clean the shelf surface with ethanol. 2. Remove the protective paper from the adhesive tape. 3. Install the holder as described in Figure 2A. (Make sure the holder is pressed against the edge of the shelf.) 4. Place the pipette onto the holder as shown in Figure 2B.
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4.PIPETTE COMPONENTS
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5.PIPETTE OPERATION
5.1Volume setting
The volume of the pipette is clearly shown through the handle grip window. The delivery volume (variable volume pipette only) is set by turning the thumb button clockwise is anticlockwise. When setting the volume, please make sure that: The desired delivery volume clicks into place The digits are completely visible in the display window The selected volume is within the pipette’s specified range Using excessive force to turn the push button outside the range may jam the
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mechanism and damage the pipette.
5.2Range Lock
Press the range lock to lock the range.
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Locked

Adjustable

One Key Range Lock: Press down the range lock to lock the range; lift up the range lock to unlock the range. Prevent pipetting errors caused by touching the adjusting knob by mistake.

5.3Sealing and ejecting tips
Before fitting a tip makes sure that the pipette tip cone is clean. PreFigs.s4 the tip on the cone of the pipette firmly to ensure an airtight seal. The seal is tight when a visible sealing ring forms between the tip and the black tip cone.

Each pipette is fitted with a tip ejector to help eliminate the safety hazards associated with contamination. The tip ejector needs to be pressed firmly downwards to ensure proper tip ejection. Make sure the tip is disposed of into a suitable waster container.

6.PIPETTING TECHNIQUES

6.1Forward pipetting
Make sure that the tip is firmly attached to the tip cone. For best results the thumb button should be operated slowly and smoothly at all times, particularly with viscous liquids. Hold the pipette vertically during aspiration. Make sure that the liquid and container vessel are clean and that the pipette, tips and the liquid are at the same temperature.
1. Depress the thumb button to the first stop . 2. Place the tip just under the surface of the liquid (2-3mm) and smoothly release
the thumb button. Carefully withdraw the tip from the liquid, touching against the edge of container to remove excess. 3. Liquid is dispensed by gently depressing the thumb button to the first stop. After a short delay continue to depress the thumb button to the second stop . This procedure will empty the tip and ensure accurate delivery. 4. Release the thumb button to the ready position .If necessary change the tip and continue with pipetting.
6.2Reverse pipetting
The reverse technique is suitable for dispensing liquids that have a tendency to
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foam or have a high viscosity. This technique is also used for dispensing very small volumes when it is recommended that the tip is first primed with the liquid before pipetting. This is achieved by filling and emptying the tip.
1. Depress the thumb button all the way to the second stop. Place the tip just under the surface of the liquid (2-3mm) and smoothly release the thumb button.
2. Withdraw the tip from the liquid touching against the edge of the container to remove excess.
3. Deliver the preset volume by smoothly depressing thumb button to the first stop. Hold the thumb button at the first stop. The liquid that remains in the tip should not be included in the delivery.
4. The remaining liquid should now be discarded with the tip or delivered back into the container vessel.
7.PIPETTING RECOMMENDATIONS
Hold the pipette vertically when aspirating the liquid and place the only a few
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millimeters into the liquid Prerinse the tip before aspirating the liquid by filling and emptying the tip 5 times.
This is important especially when dispensing liquids which have a viscosity and density different from water Always control the push button movements with the thumb to ensure consistency When pipetting liquids at a temperature different from ambient, prerinse the tip several times before use.
8.STORAGE
When not in use it is recommended that your pipette is stored in a vertical position.
9.PERFORMANCE TEST AND RECALIBRATION
Each pipette has been factory-tested and certified at 22 according to ISO8655/DIN12650. Note: Pipette specifications are guaranteed only with
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manufacturer’s tips.
9.1Performance test
Weighting should take place at 20-25, constant to ±0.5. Avoid drafts. 1. Set the desired testing volume of your pipette. 2. Carefully fit tip onto the tip cone. 3. Prerinse tip with distilled water by pipetting the selected volume 5 times. 4. Carefully aspirate the liquid, keeping the pipette vertical. 5. Pipette distilled water into a tared container read the weight in mgs. Repeat at
least five times and record each result. Use an analytical balance with a readability of 0.01 mgs. To calculate the volume, divide the weight of the water by its density (at 20: 0.9982). This method is based on ISO8655/DIN12650. 6. Calculate the F-value by using the following equation: F=inaccuracy (L) +2×imprecision (L) Compare the calculated F-value to the corresponding Fmax user. If it falls within the specifications, the pipette is ready for use. Otherwise check both your accuracy and, when necessary, proceed to recalibration procedure.
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9.2Recalibration procedure
1) Pull out the ejector button, use the calibration tool to push off the ejector button cover, and then install the ejector button back to the handle; 2) Insert the calibration tool into the button hole of the ejector, and press the calibration tool down and hold; 3At this time, it can be calibrated by turning the adjusting knob (the counter is unchanged at this time); turning clockwise to decrease the volume of liquid, and turning counterclockwise to increase the volume of liquid 4Retest after adjustment, and the pipetting volume can be within the allowable system error range.
10.MAINTENANCE
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To maintain the best results from your pipette each unit should be checked every day for cleanliness. Particular attention should be paid to the tip cone (s).
The pipettes have been designed for easy in-house service. However, we also provide complete repair and calibration service including a service report and performance certificate(s). Please return your pipette to your local representative for repair or recalibration. Before returning please make sure that it is free from all contamination. Please advise our Service Representative of any hazardous materials which may have been used with your pipette. Note: Check the performance of your pipette regularly e.g. every 3 months and always after in-house service or maintenance.
10.1Cleaning your pipette
To clean your pipette using ethanol and a soft cloth or lint-free tissue. It is recommended to clean the tip cone regularly.
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10.2In-house maintenance
1) Pull down the ring tube 2) Unscrew the nozzle connector directly counterclockwise 3) Remove the piston and spring 4) Wipe the piston and nozzle connector with alcohol and a lint-free
cloth 5) Before installing nozzle connector it is recommended to grease
the Y-shaped sealing ring slightly using the silicone grease provided. Note: Excessive use of grease may jam the piston. 6) After reassembling use the pipette (without liquid) several times to make sure that he grease is spread evenly. 7) Check the pipette calibration.
11TROUBLE SHOOTING
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Trouble

Possible cause

Solutions

Droplets left inside Unsuitable tip

Use original tips

the tip

Non-uniform wetting of the Attach new tip

plastic

Tip incorrectly attached

Attach firmly

Unsuitable tip

Use original tips

Foreign particles between tip Clean the tip cone, attach

and tip cone

new tip

Leakage

or Instrument contaminated or Clean and grease Y-

pipetted volume too insufficient amount of grease shaped sealing ring, clean

small

on Y-shaped sealing ring

the tip cone Grease

accordingly

Incorrect operation

Follow instruction carefully

Calibration altered or Recalibrate according to

unsuitable for the liquid

instructions

Instrument damaged

Send for service

Push

button Piston contaminated

Clean and grease Y-

jammed or moves

shaped sealing ring, clean

erratically

the tip cone

Penetration of solvent Clean and grease Y-

vapours

shaped sealing ring, clean

the tip cone

Pipette blocked Liquid has penetrated tip cone Clean and grease Y-

aspirated volume and dried

shaped sealing ring, clean

too small

the tip cone

Tip ejector jammed Tip cone and/or ejector collar Clean the tip cone and

or moves erratically contaminated

the ejector collar

12.WARRANTY INFORMATION
The pipettes are warranted for one year against defects in materials and workmanship. Should it fail to function in any period of time, please contact your local representative immediately. The warranty will not cover defects caused by normal wear or by using the pipette against the instructions gives in this manual.
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Each pipette is tested before shipping by the manufacturer. The Quality Assurance Procedure is your guarantee that the pipette you have purchased is ready for use.
13.SPECIFICATIONS

Single-channel Adjustable Volume Pipettes10 models, covering 0.1L~10mL.

Systematic Error

VoLume Range Increment Test VoLume

Random Error

2.5uL

1.4% 0.04 0.7% 0.02

0.1-2.5uL

0.002uL

1.25uL

2.5% 0.03 1.5% 0.02

0.25uL

12.0% 0.03 6.0% 0.02