GE-384G GeneExplorer Thermal Cycler
GeneExplorer Thermal Cycler
The GeneExplorer Thermal Cycler is a laboratory instrument
manufactured by Hangzhou Bioer Technology Co., Ltd. It is available
in different models: GE-96G, GE-384G, GE-4I, GE-48D, GE-48DS,
GE-48DG, and GE-96S.
Important Notes
Please read the following important information before using the
GeneExplorer Thermal Cycler:
- Modification to the information contained herein may occur
without further notice. - The document contains patent information under copyright
protection. All rights reserved. - The Operation Manual should be carefully read before using the
instrument for the first time.
Safety
Ensure the following safety precautions when using the
GeneExplorer Thermal Cycler:
- Instrument grounding: The instrument must be reliably
grounded to prevent electric shock. It uses a three-core grounding
plug that requires a grounding socket. - Keep away from energized circuit: Do not dismantle the
instrument without proper authorization. Component replacement or
adjustment should be done by specialized maintenance
personnel. - Attention for power supply: Before connecting AC power
supply, ensure the voltage consistency and the rated loading of the
power socket. - Attention for power line: Handle the power line with
care to avoid damage. - Plug in and plug off: Hold the plug when plugging in or
unplugging the instrument. Ensure proper insertion and avoid
forcibly pulling the power line. - Attention for instrument placing: Do not install the
equipment on inflammable materials. - Attention for operation: Take precautions to avoid
liquid dripping on the instrument. Wear personal protective
equipment, such as protective gloves, when handling reagents.
Note: In case of the following situations, immediately
unplug the power supply and seek assistance from the supplier or
qualified maintenance personnel:
- Liquid enters into the instrument.
- The instrument is exposed to rain or water.
- The instrument fails to work normally.
GeneExplorer Thermal Cycler Instruction for Use
GE-96G, GE-384G, GE-4I, GE-48D, GE-48DS, GE-48DG, GE-96S
Note:
Hangzhou Bioer Technology Co., Ltd.
Please understand that no further notice will be given for any
modification to the information contained herein. The document contains patent information under copyright protection. All rights reserved. Without prior written approval from Hangzhou Bioer Technology Co., Ltd., any part of the document shall not be copied, re-produced or translated into other language.
Before using the instrument for the first time, please carefully read the Operation Manual
File No.: BYQ56170000000ESM File Version: Nov. 2021 Edition 2.0
GeneExplorer Thermal Cycler Instruction for Use
Important Notes
Important Notes
Convention Attention: Information of extreme importance is contained under the item of
Attention, thus please carefully read it. Failure to practice as per the instruction may lead to equipment damage or operation failure. Warning! Information under the item of Warning requires special attention for specific operation step or method. Failure to practice as per the requirements may lead to severe personal injury.
1 Safety
The following basic safety measures must be abided by through all stages including equipment operation, maintenance and repair. Failure to abide by these measures or warnings indicated in the manual may affect the protection provided by the instrument. Meanwhile, it may damage the safety standard for design and manufacturing, and expected application scope of the instrument. Hangzhou Bioer Technology Co., Ltd. takes no responsibility for any consequences arising from user’s failure for abiding by the following requirements.
Attention: The instrument is Type-I equipment in line with GB4793.1 standard with protection level of IP20. The instrument is designed for indoor use.
A) Instrument grounding
To prevent electric shock, the input power line of the instrument must be reliably grounded. The instrument adopts three-core grounding plug which includes a third (grounding) foot and can only used together with grounding socket. This is a safety device. If the plug cannot be inserted into socket, please ask an electrician to install correct socket, and don’t let the grounding socket lose its function of safety.
B) Keep away from energized circuit
Operator should not arbitrarily dismantle the instrument. Component replacement or in-machine adjustment must be completed by specialized maintenance personnel with certificate. Don’t replace any component when the power line is connected.
C) Attention for power supply
Before connecting AC power supply, ensure the consistence of the voltage of the power supply and the voltage required by the instrument (a deviation of 10% is allowed), and ensure the
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GeneExplorer Thermal Cycler Instruction for Use
Important Notes
rated loading of the power socket is no less than the requirement of the instrument.
D) Attention for power line
Generally, the instrument should use the power line supplied along with the instrument. In case of power line damage, it must be replaced instead of repair. For replacement, power line of same type and specification should be used. Don’t put anything on the power line when the instrument is being used. Don’t place the power line in the place where people walk through. If the power line will contact thermal surface when it is used, then protection measures should be taken to prevent insulation damage to the power line.
E) Plug in and plug off
The plug must be held in hand for plugging in and plugging off. For plugging in, ensure that the plug is completely inserted into the socket, and don’t pull the power line forcibly for plugging off.
F) Attention for instrument placing
The instrument should be placed in area of low humidity, less dust and far away from water source (such as close to water pool or water pipe), and the place should be well ventilated and free from corrosive gas or high magnetic field interruption. Don’t place the instrument in moisty or dusty area. Also, don’t place the instrument in area making it difficult to operate the disconnecting device. Openings on the instrument are designed for ventilation. To avoid overheating, don’t bloeck or cover the ventilating openings. For single machine operation, the ventilating openings on the instrument should be kept at least 20cm away from nearest objects. For simultaneous multiple machine operation, the distance between instruments should be no less than 10cm. additionally, don’t use the instrument on floppy surface; otherwise the ventilating openings at instrument bottom may be blocked. Over-temperature will affect the instrument performance or lead to faulty. Don’t use the instrument under direct sunlight and keep the instrument away from heater, furnace and other heat source. When the instrument is not in use for an extended period, unplug the power supply and cover the instrument with soft cloth or plastic paper to prevent dust entering in the machine.
Warning: The equipment should not be installed on the surface of inflammable materials!
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GeneExplorer Thermal Cycler Instruction for Use
Important Notes
G) Attention for operation During experiment operation, avoid liquid dripping on the instrument. When dealing with reagent, wear personal protective equipment such as protective gloves.
Attention:
In any of the following cases, immediately unplug the power supply from the socket, and contact the supplier or ask qualified maintenance personnel for treatment: Liquid enters into the instrument. The instrument is exposed to rain or wet by water. The instrument fails to work normally, especially there is any
abnormal sound or flavor. The instrument drops down or the shell is damaged. The instrument function has evident change. For any doubt concerning the compatibility between detergent and
equipment part or materials contained in the equipment, please consult the manufacturer or its agent.
H) Re-transport If the instrument needs to be transported again, the detection hole position and the instrument should be thoroughly cleaned and sterilized with ultraviolet light before transportation. IEquipment Safety
The instrument was designed, produced, and tested in accordance with EN 61010-1 (IEC 61010-1) “Safety requirements for electrical equipment for measurement, control, and laboratory use — Part 1: General requirements”. It has left the factory in a perfectly safe condition. The instrument meets the requirements of the Regulation (EU) 2017/746 of the European Parliament and of the Council of 5 April 2017 on in vitro diagnostic medical devices. 2 Instrument label
A) Warning Sign Warning identification
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GeneExplorer Thermal Cycler Instruction for Use
DANGER!
SCALDING!
BIOHAZARD PROTECT CONDUCTOR TERMINAL
Warning mark
Important Notes
Area with the mark pasted on the instrument shall avoid improper use and be careful of danger. Area with the mark pasted on the instrument causes high temperature and is scalding during use. Area with the mark pasted on the instrument will caused biohazard during use. PROTECT CONDUCTOR TERMINAL is near to the area with the mark pasted on the instrument
Warning! The instrument is pasted with a label of “HOT SURFACE!”. Any part of body is strictly prohibited from touching the metal part (module and hot lid) around the label during instrument operation or within a certain period after the program ends.
B) Other symbols on the packaging
Date of manufacture
RoHS Consult instructions for use Serial number
Catalogue number In vitro diagnostic medical device CE mark Manufacturer
Indicates the date when the medical device was manufactured.
Restriction on the use of certain hazardous materials(restriction of hazardous substances)
Indicates the need for the user to consult the instructions for use.
Indicates the manufacturer’s derail number so that a specific medical device can be identified. Indicates the manufacturer’s catalogue number so that the medical device can be identified. Indicates a medical device that is intended to be used as an in vitro diagnostic medical device.
Indicates the medical device meets the CE related Directives.
Indicates the medical device manufacturer.
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GeneExplorer Thermal Cycler Instruction for Use
Important Notes
Authorised representative in the European Community Up
Fragile
Keep dry The limit of stacking layer
Temperature limit
3 Instrument calibration
Indicates the authorized representative in the European Community.
Indicates that the correct position of the transport package is vertical upward. The transport packages contain fragile goods, so they should be handled with care.
The package should be rain-proof.
Maximum stacking layer of the same package is 2. Indicates that the temperature limit of transportation package should be – 20 to 55 .
Before the instrument is delivered, the device is fully calibrated according to the Bioer ‘s factory inspection standard, all the actions are under the requirements of ISO 13485 Medical devices Quality management systems Requirements for regulatory purposes. If the instrument is installed in the required working environment, there is no need to calibrate or correct the temperature after installation.
4 Instrument maintenance
Periodically use clean, soft cloth dipped with ethanol to clean the block wells on module to ensure sufficient contact between test tubes and taper holes and to ensure favorable heat conduction. If there is any dirt on the instrument surface, use soft cloth dipped with cleansing cream to clean.
Warning! The power must be cut off when cleaning the instrument surface. Corrosive detergent is strictly prohibited from being used to clean the instrument surface. The instrument module includes precise optics, dust, foreign matter and residue should be avoided.
5 Disposal
Potentially infectious material and all parts that may come in contact with potentially infectious material must be disposed in accordance with the relevant legal provisions.
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GeneExplorer Thermal Cycler Instruction for Use
Important Notes
All parts which have been replaced must be disposed in accordance with the relevant legal provisions.
Disposal of the instrument must be carried out in accordance with the relevant legal provisions. Disposal of the packaging material must be carried out in accordance with the relevant legal provisions.
6 After-sales service
See the Warranty Card for specific content and scope of warranty. Attention: After opening the packaging box, immediately check the objects contained therein based on the packing list. In case of any object damage or missing, please immediately contact the supplier. After acceptance inspection, fill out the Acceptance Inspection Form and send the copy (or fax) back to the company for filing and warranty service. After the packaging box is opened, please properly keep the packaging box and packing materials which will be used during maintenance. Hangzhou Bioer Technology Co., Ltd. will take no responsibility for instrument damage caused by improper packaging during transport to the maintenance department.
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GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
Contents
CHAPTER ONE INTRODUCTION …………………………………………………………………………………………………………3
1 INTRODUCTION TO PCR TECHNOLOGY …………………………………………………………………………………………..3 2 EXAMPLES OF PCR’S APPLICATIONS ………………………………………………………………………………………………3 3 FEATURES OF GENEEXPLORER THERMAL CYCLER …………………………………………………………………………..3 4 USE OF GENEEXPLORER THERMAL CYCLER……………………………………………………………………………………4 5 SERVICE LIFE OF THERMAL CYCLER ………………………………………………………………………………………………5
CHAPTER TWO CHARACTERISTICS ………………………………………………………………………………………………….6
1 NORMAL OPERATION CONDITIONS …………………………………………………………………………………………………6 2 TRANSPORT AND STORAGE CONDITION …………………………………………………………………………………………..6 3 BASIC PARAMETERS…………………………………………………………………………………………………………………….6 4 SOFTWARE FUNCTIONS ………………………………………………………………………………………………………………..7 5 SOFTWARE VERSION ……………………………………………………………………………………………………………………7
CHAPTER THREE PREPARATIONS……………………………………………………………………………………………………..8
1 STRUCTURAL SCHEMATIC DIAGRAM ………………………………………………………………………………………………….8 2. CHECK BEFORE TURNING ON THE INSTRUMENT ……………………………………………………………………………………9 3 TURN ON THE INSTRUMENT………………………………………………………………………………………………………………..9
CHAPTER FOUR OPERATION INSTRUCTIONS ………………………………………………………………………………..10
1 TURN ON THE INSTRUMENT………………………………………………………………………………………………………………10 2 PCR PROGRAM EDITING…………………………………………………………………………………………………………………..12 2.1 SETTING OF PROGRAM SECTION ……………………………………………………………………………………………………..13 2.2 SECTION TEMPERATURE AND CONSTANT TEMPERATURE TIME ……………………………………………………………..13 2.3 FUNCTION OF LOOP………………………………………………………………………………………………………………………14 2.4 EXTENDED PARAMETER ………………………………………………………………………………………………………………..15 2.5 SAVE EXPERIMENT ……………………………………………………………………………………………………………………….16 3 RUN PROGRAM ………………………………………………………………………………………………………………………………16 3.1 TEMPERATURE CONTROL MODE ……………………………………………………………………………………………………..17 4 FILE MANAGEMENT ………………………………………………………………………………………………………………………..18 5 SYSTEM SETTINGS ………………………………………………………………………………………………………………………….20 5.1 PERSONAL CENTER………………………………………………………………………………………………………………………21 5.2 SYSTEM SETTINGS ……………………………………………………………………………………………………………………….21 6 SOFTWARE UPGRADE ………………………………………………………………………………………………………………………21 6.1 UI PROGRAM UPGRADE…………………………………………………………………………………………………………………21 6.2 DRIVER UPGRADE ………………………………………………………………………………………………………………………..22
CHAPTER FIVE FAULT ANALYSIS AND TROUBLESHOOTING ……………………………………………………….23
1 FAULT ANALYSIS AND TROUBLESHOOTING ………………………………………………………………………………………….23 APPENDIX 1 WIRING DIAGRAM (THE DIAGRAM IS ONLY FOR REFERENCE, AND PLEASE UNDERSTAND THAT NO
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GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
FURTHER NOTICE WILL BE GIVEN FOR ANY MODIFICATION)………………………………………………………………………25 APPENDIX 2 VALUE AND MEANING OF SET PARAMETERS…………………………………………………………………….26 APPENDIX 3 PRODUCT EMC STATEMENT ……………………………………………………………………………………………..28 APPENDIX 4 LIST OF ACCESSORIES………………………………………………………………………………………………………30
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GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
Chapter One Introduction
The chapter covers introduction to PCR technology and examples of its applications, introduction to PCR Thermal Cycler and features of GeneExplorer Thermal Cycler.
1 Introduction to PCR technology
Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro simulation of natural DNA reproduction process. With two DNA chains to be amplified as a template and mediated by a pair of artificially synthetic ODN primers, and through DNA polymerase of high temperature resistance, it can rapidly and specifically amplify specific DNA segment. Due to its simplicity, rapidity, peculiarity and sensitivity, ever since invention of PCR by Mullis in 1985 and discovery of DNA polymerase of high temperature resistance by Erlich in 1988, over a few years, the technology has been widely applied in various fields of bioscience. Especially in cytology, virology, oncology, genetic epidemiology, forensic pathology and flora and fauna immunology, it has accomplished outstanding achievements and become a milestone of contemporary molecular biology development.
2 Examples of PCR’s applications Application of Thermal Cycler: The product is applicable to polymerase chain reaction in fields including immunology, human genome engineering, forensic pathology, oncology, organization and population biology, paleontology, zoology and botany.
3 Intended use
The GeneExplorer Thermal Cycler is an automated instrument used for qualitative amplify the related nucleic acids in human sample using the polymerase chain reaction process. The instrument is for in vitro diagnostic only. The GeneExplorer Thermal Cycler is intended for used in medical and biological laboratories by professional user trained in molecular biological techniques and the operation of GeneExplorer Thermal Cycler.
4 Features of GeneExplorer Thermal Cycler
GeneExplorer Thermal Cycler is a PCR of multiple functions and applications. It features as below: Providing up to eight replaceable modules based on requirements of various PCR
experiments.
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GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
Adopting advanced American ITI thermoelectric refrigeration technology and thermoelectric refrigeration element manufactured under Japanese quality management mode which presents stable and reliable performance;
Maximum ramp rate up to 4C/s and can be adjusted based on experiment requirements. Multiple temperature control modes and higher reagent compatibility. Host machine of GeneExplorer Thermal Cycler can automatically recognize module and
run corresponding program. Colorful large-screen display, humanized operation interface, simple and clear operation. Power-off protection function, and after power supply recovery, it can continue to complete
the program unfinished. Hot lid can adjust height and pressure based on actual using condition to adapt to different
reaction tubes and effectively prevent reagent evaporation and pollution. USB standard port can be connected to general computers, convenient for software upgrade.
5 Use of GeneExplorer Thermal Cycler
GeneExplorer Thermal Cycler, taking a leading position among similar products, has combined various advanced technologies and can meet requirements of various PCR experiments. Different modules have the suitable consumables, there are as follows.
Standard 0.2ml centrifuge tube
GE-96G / GE-96S: (1) 1×96×0.2ml 0.2ml standard centrifuge tube (2) 12×8×0.02ml PCR 8-Strip Tube (3) 96×1×0.2ml (96 wells PCR Microplate)
PCR Microplate
GE-48DGE-48DG: GE-48DS: (1) 48×0.2ml+48×0.2ml (48 wells PCR Microplate)
GE-384G 384×0.02ml384 wells PCR Microplate
GE-4I 4 pcs.of glass slide
Glass slide
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GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
6 Service life of Thermal Cycler Service life of Thermal Cycler is 5 years from the date of instrument installation.
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Gene Explorer Thermal Cyeler Operation Manual
Chapter Two Characteristics
Chapter Two Characteristics
The chapter mainly introduces the operation, transport and storage conditions of the instrument, and its basic parameters, performance and functions.
1 Normal operation conditions
Ambient temperature: 10C ~ 30C RH: 70% Power supply: 220V 50Hz Altitude: 2km
Attention:
Before using the instrument, please confirm if the working conditions can meet the above requirements. Pay special attention to reliable grounding of the power line.
2 Transport and storage condition
Ambient temperature: -20C ~ +55C RH: 80%
3 Basic parameters
The parameters of the modules are as below:
Name Model
Gene Explorer Thermal Cycler GE-96G GE-384G GE-48D GE-4I GE-48DG GE-48DS GE-96S
Sample size
96×0.2ml
384×0.02ml
48×0.2ml+4 8×0.2ml
44×gla ss slide
48×0.2ml+4 8×0.2ml
48×0.2ml+4 8×0.2ml
96×0.2ml
gradient temperature range gradient temperature difference range Ladder Temperature range Energy consumption Fuse
LCD display
30~99
/
1~30
/
/
600W 250V 12A 5 20 Color touch screen (8 inch, 260,000 colors)
30~99
1~30
1200W
/ / 0.1~6
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Gene Explorer Thermal Cyeler Operation Manual
Temperature range File saving amount Operation mode Dimensions
Net weight
4.0-105°C(min. resolution: 0.1°C)
2000 standard programs
Continuous operation 420mm×269mm×254mm(L×W×H) 13kg
Chapter Two Characteristics
Attention:
When the set value of hot lid temperature is lower than the set value of module temperature, the hot lid temperature displayed on the screen will be higher than the set value of hot lid temperature.
4 Software functions
(1) file edit (including file number, temperature and time of each section, setting of loop times, view, revise and delete file, automatic file connection, gradient temperature setting; maximum number of editable sections is 10, maximum number of steps is 100, maximum number of loop is 99, available for secondary nesting, gradient temperature range is 1~30; Ladder Temperature range 0.1~6 (2) file storage. (3) file modification (including adjustment of ramp rate, automatic temperature & time modification during cycling, time display and calibration). (4) setting of temperature, time and loop times during file running, and display of instant values (including voice prompt, estimation of overall and remaining time of file running, fault protection and warning); (5) stop file running (including pause file running, stop file running and automatic recovery after outage).
Attention:
The above software functions are only for reference. Please understand that no further notice will be given for any modification to the software functions.
5 Software version
Software version: V1.0
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Gene Explorer Thermal Cyeler Operation Manual
Chapter Three Preparations
Chapter Three Preparations
The chapter mainly introduces the instrument structure, operation of touch screen, and preparations before turning on the instrument. If the instrument is used for the first time, please be familiar with the contents in the chapter before turning on the machine.
1 Structural schematic diagram
The structural parts of the instrument include heating module, hot lid, power switch module
(including power switch, power socket, and fuse holder), and micro-computer controller and
touch screen. All the modules of the instruments are the same except the heating modules,
Diagram for GE-96G, GE-96S, GE-384G
,:
4
2
5 1
3
Heating Module for GE-48DS,
6/7 Heating Module for GE-48D,GE-48DG
1
1
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Gene Explorer Thermal Cyeler Operation Manual
Chapter Three Preparations
Attmenotdiuolne: 1 Requirehmoetnltisd for fuse: 250VLC1D2sAcreen 520. For fpurseessurereplacbeamlaenncti,ng compression papdroductpwohwicehr sioscikneltine withtheporewqeurirsewmitecnhts should be used. Contact the
supplier or manufacturer if you need fuse.
2 compression pad for 96 wells PCR plate and 384 wells PCR.
3 pressure balancing plate for 0.2ml single tubes and 8-strip tubes
Usage of compression pad
Usage of pressure balancing plate
2. Check before turning on the instrument
Before plug in and energize the instrument, please confirm the following: 1 Confirm the power supply meets the voltage required by the instrument; (see Chapter
Two for power supply requirements) 2 Confirm that the power supply plug has been reliably inserted into the power socket; 3 The power line is reliably grounded.
3 Turn on the instrument
Turn on the power switch (switch to the position of “”), and then the instrument will carry out self-test which will take about 20s, please wait patiently. If the self-test has discovered no problems, the main interface will show on the screen, and you can edit, view, revise or delete PCR amplification files.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Chapter Four Operation Instructions
In this chapter, the methods of editing, viewing, revising, and deleting PCR amplification files are introduced in detail, as well as the method of running PCR amplification files and setting of instrument system parameters. Warning! After the instrument is turned on, if there is any abnormal sound or display, or
fault alarm and prompt present during instrument self-test, please immediately turn off the power and contact the supplier.
Attention:
If the sample volume of your experiment is less than the number of cone holes of the module, please evenly distribute the test tubes to ensure stable press of the hot lid on the test tube tops, which will also contribute to uniform module loading and ensure uniformity of temperature change in each test tube.
1 Turn on the instrument
Turn on the power switch and then the instrument will carry out self-test, please wait patiently. If the self-test has discovered no problems, the main interface will show on the screen. Module Type is displayed at bottom left of the main interface. At the middle of the main interface, it displays four functions of software which are New Experiment, Open Experiment, Recently Opened and Settings.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Picture for GE-96G
Picture for GE-48DS Picture of Model GE-384G/GE-4I/GE-96S would show the module type with 384G, plate and 96V respectively. Press the icon “New Experiment” to enter into the interface of experiment parameter
setting.
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Chapter Four Operation Instructions
Press the icon “Open Experiment” to enter into the interface of file list. Press the icon “Recently Opened” to view experiment. Press the icon “Settings” to enter Personal Center and System Settings.
During operation, instrument operation status is displayed on the main interface, and click to enter the interface of operation monitoring. When operation is stopped, click to enter the interface of experiment parameter setting.
2 PCR program editing
Click “New File” on the main interface or click “New Experiment” under “Open Experiment” or “Recently Opened” to enter into the interface of program setting.
Picture for “Open Experiment” or “Recently Opened” selection
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Picture for “New Experiment” selection 2.1 Setting of program section Select one section of the program and the section will light up; click “Section”, then there will prompt the checkbox of “Insert” and “Add”. Click “Insert”, a new section will be added before the section selected and lighting up. Click “Add”, a section will be added to the end of the program. Click “Delete” to delete the section selected. 2.2 Section temperature and constant temperature time On the interface of PCR program setting, click the small icon on upper right of the screen, and it will prompt the temperature and time setting box for setting temperature and constant temperature time of the program section.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Run mode: Standard Mode and High-speed Mode. Standard Mode running characteristics simulate BIOER TOUCH series.
2.3 Function of loop
On the interface of PCR program setting, click “Loop”, and it will prompt interface 1-1, select the starting and ending sections of the loop, click “Determine” and it will prompt the setting box 1-2 for loop times, input the times and click “OK”, the setting of loop section will be finished. Attention: In one program, multiple loop sections can be set, but these sections cannot
be crossed, but only paralleled or included.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Interface 1-1
Interface 1-2 2.4 Extended parameter On the interface of PCR program setting, click “Extended parameter” and it will prompt a box for setting extended temperature, extended time, speed, and gradient and other operations. Extended temperature: the volume of temperature increase or decrease for each loop. “-/+” changes the positive and negative of data.
Extended time: time increase for each loop. Speed: ramp rate
Gradient: gradients temperature refers to the temperature difference between the most left and most right ranks of the module. In “Temperature”, the temperature of the most left rank is set. GE-48DG, GE-96G, GE-384G own this function. Ladder Temp: It refers to the temperature difference between two adjacent modules. The range is 0.1~6. “Temperature” is set to the temperature of the leftmost module. GE-48DS, GE-96S own this function.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
2.5 Save experiment On the interface of PCR program setting, click “Save”, and input username and file name to save the file. Files saved can be found under “Open Experiment”. 3 Run program Before running experiment, set up hot lid temperature, reaction volume, temperature control mode and control mode on the interface of PCR program setting and then click “Run” to start the experiment. You can also run experiment by directly selecting the file under “Open Experiment” and “Recently Opened”.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Click
it will display the temperature of each module.
3.1 Temperature control mode “Control Mode” represents the default temperature control mode after the system is turn on,and it includes two modes of “Block” and “Tube”. “Block” mode only considers the temperature control of module during temperature ramp. As
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
the temperature ramp of reagent is lagging that of module, the actual temperature change of reagent is inconsistent with our settings that the actual time of reagent reaching set temperature is shorter than the set time (see the figure below for the relation between module temperature and reagent temperature).
Module Reagent
“Tube” mode considers the lagging reagent temperature ramp and provides an overshoot process after reaching set temperature to enable reagent to reach the set temperature more quickly. If setting time equal to that of “Block” mode, the actual time for reagent reaching set temperature is obviously extended (see the figure below for the relation between module temperature and reagent temperature).
Module
Reagent
4 File management
Click Open Experiment on the main interface to enter the interface of file management. Click the dropdown list on the upper left to select disk for operation. For example, select USB flash disk to operate on USB flash disk contents/GeneExplorer. Press “New Experiment” to enter the interface of experiment parameter setting. Press “Run” to enter the interface of run. Press “Export” to export file or user. Press “Function” to delete and rename file or user.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
New Experiment
Click
to enter into user management.
Press “Function” to delete or rename user. Select user to export to USB flash disk. Click “Manage File”
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
Press “Function” to delete or rename file. Select file to export to USB flash disk.
5 System settings
Click “System Settings” on the main interface to enter the interface of setting which includes two major functions which are System Settings and Personal Center.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
5.1 Personal Center
In Personal Center, you can log on with registered account, or log out.
5.2 System Settings
System Settings include the following 10 settings: 1. Network setting 2. Http remote access 3. Hot lid related 4. Time and date: set instrument time, and it will display on the lower right corner of the main interface 5. Instrument part use time 6. Dairy 7. About instruments 8. Tm calculation 9. Buzzer setting 10. Exit: exit system software
6 Software upgrade
6.1 UI program upgrade Copy file to USB flash disk versionupdate Enter into System Settings->about instruments, select Firmware upgrade/
For setting menu, please pay attention to the capitalization of letters.
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GeneExplorer Thermal Cycler Operation Manual
Chapter Four Operation Instructions
6.2 Driver upgrade File Copy to USB flash disk /GeneExplorer location Enter into System Settings->about instruments, select driver upgrade/
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Gene Explorer Thermal Cycler Operation Manual
Chapter Five Fault Analysis and Troubleshooting
Chapter Five Fault Analysis and Troubleshooting
The chapter mainly introduces possible faults of the instrument, cause analysis and troubleshooting. 1 Fault analysis and troubleshooting
No. Fault
Cause analysis
Troubleshooting
Disconnecting power supply
Check and connect the power supply
1
After the power switch is turned on, the display
Burned fuse
Replace fuse (250V 12A 5×20)
screen doesn’t work.
Damaged switch
Replace switch
Others
Contact supplier or manufacturer
2
During file running, it
shows “Err code:
0x**0000***,
and
prompts “Please Contact
the Manufacturer”
Module sensor damage or loose contact Radiator sensor damage or loose contact Hot lid sensor damage or loose contact
Contact supplier manufacturer
or
Remove the blocking
Blocked ventilation holes
objects on ventilation
holes
Evident change of
Open the shell and check
3
module temperature rise rate or imprecise Loose connecting line
the connecting line; tighten the screw of the
temperature control
terminal block if it is
loose.
Damaged TEC
Contact supplier or
manufacturer
Evident slowing module Ambient temperature or humidity is Please properly adjust
temperature fall rate or too high, and exceeds using ambient temperature and
4 failure to fall below condition of the instrument
humidity
ambient temperature
Damaged TEC
Contact supplier or
Damaged or un-working fan
manufacturer
5
Temperature changing
not Damaged temperature sensor Damaged TEC
Contact supplier or manufacturer
On system parameter setting Set the hot lid status as
interface, hot lid status is set as specific
temperature
“OFF”.
value
6
The module fails to heat
Temperature control time on file editing interface is set as “–:–“, Set temperature control
leading to automatic turning off of time in digital form
hot lid
Loose connector
Contact supplier or
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Gene Explorer Thermal Cycler Operation Manual
Chapter Five Fault Analysis and Troubleshooting
Damaged heating component in hot
lid
Damaged temperature sensor in hot
lid
7
Abnormal characters Loose contact of PCB
displayed on screen
Damaged PCB
Hot lid temperature is not set, hot lid is set as “OFF”.
8
Reagent evaporation in reaction tube
Uneven tubes
distribution
of
reaction
Untightened PCR tube caps Explanation for Err code: 0x**0000*** is as below:
manufacturer
Contact supplier or manufacturer When setting parameters for running file, select ON for hot lid control and set proper temperature Adjust reaction tube distribution to ensure symmetricity. Close reaction tubes tightly and then put into the instrument
0X Left Right Reserve Reserve Reserve Reserve Single module Double-modul Radiato
sensor sensor d
d
d
d
hot
e hot lid B
r
lid/double-modu
le hot lid A
0 normal 1 open circuit alarm 2 short circuit alarm/over-temperature radiator alarm
Attention:
1. During the warranty period, user is strictly prohibited from opening
the shell of the instrument for inspection. In case of any fault
requiring opening the shell for inspection, user should timely contact
supplier or manufacturer.
2. As temperature control features (including ramp rate, stability and volatility) differ for various brands and instruments, also due to the uncertainty of bio-experiments which can be easily affected by external environment, PCR program of successful running on one instrument may not achieve the equal effect on another. Therefore, when you are changing an instrument, you may need to adjust the PCR running program for an ideal effect.
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Gene Explorer Thermal Cycler Operation Manual
Appendix 1
Appendix 1 Wiring Diagram (the diagram is only for reference, and please understand that no further notice will be given for any modification)
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Gene Explorer Thermal Cycler Operation Manual
Appendix 2 Value and Meaning of Set Parameters
Appendix 2
No. Parameter Meaning
Set value range
Remark
1 Temp(C)
Temperature control point (C)
4.0~99.0
2 Time
Temperature control time (mm:ss)
–:–
(press “+/-” key before “:” to set)
Infinite temperature control time and turn off hot lid in the meantime
00:00~59:59
GE-48DG,GE-48DS,
GE-96S: 0.1-6.0
Temperature heating GE-96G,GE-48D:0.1-5.
3 Ramp(C/S)
and cooling rate 0
(C/s)
GE-384G,GE-4I:0.1-4.
0
#.#
Max. ramp rate
Temperature
4 +Temp
increase for each 0.0~9.9
cycle (C)
5 +Time
Time increase for each cycle (m:ss)
0:00~9:59
Cycle1
Number of the cycle 1~5
×00
Times of the cycle 1~99
6 From 00
Starting section of X(any existing section Cycle1:×00 From 00 to
the cycle
number)
00
to 00
Ending section of the cycle
X+15
7 Grad.
Gradient temperature (C)
0 ~30
For GE-48D, GE-4I, do not have the parameters.
8 Date
Date (YY-MM-DD)
00(~99)-01(~12)-01(~3 1)
YY-MM-DD
9 Time
Time (HH:MM:SS) 00:00:00~23:59:59
HH:MM:SS
10 Default File
System name
default
file
Max. 11 characters
11
Default User
System default user name
Max. 11 characters
In this mode (enter into constant temperature status after reaching set
12
Control Mode
Temperature control mode
Block
temperature), the time for reagent at set
temperature is less than
the set time (see Fig. 1).
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Gene Explorer Thermal Cycler Operation Manual
Tube
13
Sample Volume
Sample volume (ul) 10~200
14 Holid
Hot lid off Hot lid on ()
Off 30~110
15 Key Sound Key sound (yes/no) Yes/No
16
Run Sound
End Run end (yes/no)
sound Yes/No
Module Reagent
Appendix 2
In this mode (enter into
constant temperature
status when overshoot
for about 3 after
reaching
set
temperature), reagent
temperature can get
close to the set
temperature
more
quickly (see Fig. 2).
Buzz Intermittent buzz
Module Reagent
1
2
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Gene Explorer Thermal Cycler Operation Manual
Appendix 3
Appendix 3 Product EMC Statement
Class-I Type-A Equipment If the instrument is used under dry environment, especially under dry environment where artificial materials (artificial fabrics, carpet, etc.) present, electrostatic discharge may be generated which may lead to false conclusion. The instrument is strictly prohibited from using near strong radiation source; otherwise, normal equipment operation may be interrupted. EMC should be in line with the requirements of GB/T 18268.1-2010 and GB/T 18268.26-2010. 1. Requirements for immunity:
Table 1 Requirements for equipment immunity
Port Shell
AC
DCc I/O signal b
Test item
Electrostatic discharge (ESD) Radiation magnetic field Rated PFMF a
Voltage sag d
Voltage interruption d Pulse group
Surge
Radio frequency transmission
Pulse group
Surge
Radio frequency transmission
Pulse group
Surge
Radio
EMC basic standard
Test value
Performance criterion
GB/T 17626.2
Air discharge:2kV,4kV,8kV; Contact discharge:2kV,4kV
B
GB/T 17626.3
3V/m,80MHz-2.0GHz,80% AM
A
GB/T 17626.8 3A/m,50Hz
A
1 cycle 0%;
B
GB/T 17626.11 5 cycle 40%
C
25 cycle 70%
C
GB/T 17626.11 5%, duration:250 cycle
C
GB/T 17626.4 GB/T 17626.5
1kV(5/50ns.5kHz)
B
Line to ground:2kV/ Line to ground:1kV
B
GB/T 17626.6
3V,150kHz-80MHz,80%A M
A
GB/T 17626.4 GB/T 17626.5
1kV(5/50ns.5kHz)
Line to ground:2kV/ Line to ground:1kV
Not applicable Not applicable
GB/T 17626.6
3V,150kHz-80MHz,80%A M
Not applicable
GB/T 17626.4 0.5kV(5/50ns.5kHz) GB/T 17626.5 / GB/T 17626.6 3V,150kHz-80MHz,80%A
Not applicable Not applicable Not
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Gene Explorer Thermal Cycler Operation Manual
Appendix 3
frequency
M
transmission
I/O
Pulse group GB/T 17626.4 1kV(5/50ns.5kHz)
signal to main
Surge
GB/T 17626.5 /
power supply
Radio frequency transmission
GB/T 17626.6
3V,150kHz-80MHz,80%A M
A The test is applicable only to equipment potentially sensitive to
magnetism. CRT display interruption value is allowed to be higher than
1A/m.
B Only applicable to cables longer than 3m.
C Not applicable to input port expected to connect to battery or
chargeable battery (before recharging, it should be removed or
disconnected from the equipment). For equipment with DC power input
port (using AC-DC power adapter), test should be conducted on AC input
port of AC-DC power adapter specified by the manufacturer. If there is no
specific requirement, typical AC-DC power adapter should be adopted. The
test is applicable to DC power input port designed for extended long
distance connection.
D “5/6cyle” refers to “5 cycles under 50Hz test” and “6 cycles under 60Hz
test”.
applicable
Not applicable Not applicable
Not applicable
2. Requirements for radiation:
Table 2 Requirements for equipment radiation
Radiation test
Compliance
Radio-frequency emission GB 4824
Class 1
Radio-frequency emission GB 4824
Type A
The equipment is designed and tested based on Type-A equipment in GB4824. In family
environment, the equipment may cause radio interference, and thus protective measures should
be taken.
Electromagnetic environment evaluation is suggested before the equipment use.
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Gene Explorer Thermal Cyeler Operation Manual
Appendix 4
Appendix 4 List of Accessories
No. Name
Model Specification
Power line (with shield
1
250V10A
magnetic ring clamp)
& Unit QTY Remark
String 1
Accessory
2 Fuse
250V12A, 5×20 Piece 2
Accessory
3 WiFi Module
Piece 1
Accessory
4 Ethernet Patch Cable
Piece 1
Accessory
5 Compression Pad Pressure Balancing
6 Plate
Piece 2 Piece 1
Accessory Accessory
30
Hangzhou Bioer Technology Co., Ltd.
Hangzhou Bioer Technology Co., Ltd. Add: 1192 BinAn Rd, Binjiang District, 310053 Hangzhou,
PEOPLE’S REPUBLIC OF CHINA Website: www.bioer.com.cn Tel:+86-571-8580053587774558 Fax:+86-571-8580053787774559 E-mail: [email protected]
MedNet EC-REP GmbH Borkstrasse 10, 48163 Muenster, Germany
杭州博日科技股份有限公司
