Hygiena™ Prep Xpress Consumables Guide
Hygiena™ Prep Xpress
User Guide
Hygiena’s modular Prep Xpress provides liquid handling automation for a variety of PCR processes.
The Consumables Guide below details the required, protocol-specific accessories.
Prep Xpress Consumables Guide
| Protocol | Accessory | Description | Total | SKU |
| All Protocols | Plate | Hygiena Cluster Tube Green Rack Adapter, 1.1 mL | 10 (10 included in startup pack) | MIS2103 |
| Prep-Lysis Solution (6 racks) | Reservoir | Vblock 200 mL Open Reservoir, V-bottom, Clear PP | 1 | MIS2104 |
| Tips | 300 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case) | Dependent on Volume | MIS2082 | |
| Prep-BHI Solution (5 racks) | Reservoir | Seahorse-Agilent, Reservoir, 1 cavity, 8 rows, 290 mL | 1 | MIS2111 |
| Tips | 1,000 µL Framed Conductive Filtered (5 racks per sleeve – 8 sleeves per case) | Dependent on Volume | MIS2108 | |
| Transfer-5 uL Cluster Tube to Cluster Tube Transfer-20 uL Cluster Tube to Cluster Tube | Pedestal | Conductive Tip Pedestal | 1(1 included in startup pack) | MIS2113 |
| Tips | 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case) | Dependent on Volume | MIS2107 | |
| Hydrate-30 uL-Lysate to PCR Hydrate-50 uL-Lysate to PCR Hydrate-30 uL-Lysate to PCR SINGLE Hydrate-50 uL-Lysate to PCR SINGLE | Racks | Eppendorf PCR Cooler, 200 µl, Strip-Well, PCR, White Well | 2 | MIS2109 |
| Tips | 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case) | Dependent on Volume | MIS2107 | |
| Pedestal | Conductive Tip Pedestal | 1(1 included in startup pack) | MIS2113 |
Hygiena™Prep Xpress
Tip Utilization Guide
Hygiena’s modular Prep Xpress provides liquid handling automation for a variety of PCR processes utilizing protocol-specific tips. The Tip Utilization Guide below details the tip volumes, applications, and throughput recommendations.
| Tip Volume | 50 µL (MIS2107) |
| Protocol | Transfer-Cluster tube to Cluster tube & Hydrate-Lysate to PCR |
| Tips Per Sleeve | 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips) |
| Sleeve Throughput | 1 Rack (96 tips ) = 96 samples (1 rack transfer or hydrate) |
| 1 Strip (8 tips) = 8 samples | |
| 1 Sleeve (480 tips) = 480 samples (5 racks transfer or hydrate) | |
| Tips Per Case | 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in 8×12 strips |
| Case Throughput | 1 Case = 5,760 samples (60 racks transfer or hydrate) |
| Tip Volume | 300 µL (MIS2082) |
| Protocol | Prep-Lysis Solution (6 racks) |
| Tips Per Sleeve | 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips) |
| Sleeve Throughput | 1 Strip (8 tips) = 576 samples (6 racks lysis buffer) |
| 1 Rack (96 tips ) = 6,912 samples (72 racks lysis buffer) | |
| 1 Sleeve (480 tips) = 34,360 samples (360 racks lysis buffer) | |
| Tips Per Case | 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in 8×12 strips |
| Case Throughput | 1 Case = 414,720 samples (4,320 racks lysis buffer) |
| Tip Volume | 1000 µL (MIS2108) |
| Protocol | Prep-BHI Solution (5 racks) |
| Tips Per Sleeve | 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips) |
| Sleeve Throughput | 1 Strip (8 tips) = 480 samples (5 racks BHI solution) |
| 1 Rack (96 tips ) = 5,760 samples (60 racks BHI solution) | |
| 1 Sleeve (480 tips) = 28,000 samples (300 racks BHI solution) | |
| Tips Per Case | 1 Case (3,840 tips) = 8 sleeves of 5 racks of 96 tips in 8×12 strips |
| Case Throughput | 1 Case = 230,400 samples (2,400 racks BHI solution) |
For additional Prep Xpress protocol information or technical support contact: diagnostics.[email protected]
For pricing or ordering information, contact your local Hygiena customer service or sales representative, or contact us on our website.
www.hygiena.com
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