Co-dx Vzdc-r-001 Vector Smart Zika Dengue And Chikungunya Ruo User Guide

Co-Dx VZDC-R-001 Vector Smart Zika Dengue and Chikungunya RUO

Plate the Master Mix

• Load the Co-Dx Box software.
• In the Clean area, clean work surfaces with water > 10% bleach > water > 70%alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
• Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
• Briefly vortex and centrifuge the MM and NFW reagents.
• Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
• Pipet 5 µL of NFW into only the NC well and cap the NC well. Place reagents back into the freezer.

Plate Samples and Positive Control (PC)

• Load the Co-Dx Box software.
• In the Positive area, clean work surfaces with water > 10% bleach > water > and 70% alcohol (in that order).
  
• Thaw the PC on ice.
• Briefly vortex and centrifuge all reagents.
• Perform sample extraction, either using RNA extraction or lysis.
• Add 5 µL of the sample extract to the appropriate wells and cap the sample extract wells.
• Add 5 µL PC to the PC well and cap the PC well.
• Place capped tubes in the thermocycler, in appropriate locations and fill the rest of the carousel with blank tubes.
• (Optional): Cap all tubes and place sam-ples and reagents back into the freezer.

Start the Run

• In the Co-Dx Box software, click New.Select an assay template and click OK.
• Under Run Setup, select Samples and type sample information in the appropriate order.
• Click Information and in the Notes field, type any needed information.
• Select the appropriate thermocycler and from the drop-down select Start Run.
• Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start.

The run initializes and a progress bar gives an estimated time to completion.